Перспективы использования кольцевых молекул ДНК (TREC) для оценки Т-клеточного звена иммунитета пациентов с первичным иммунодефицитом

Abstract

This article deals with the quantification assay of TREC copy number by RQ-PCR to assess the functioning of T-cell immunity. TRECs are T-cell receptor excision circles which are formed during T-cell receptor gene rearrangement process. They determine early forms of T-cells. We found that only recent thymic emigrants (CD4+CD45RA+CD31+) and naive T-cells (CD4+CD45+CD31-) contain TREC. We analysed 44 patients with different primary immune disorders (PID). It was found out that patients with PID have dramatically decreased level of TRECs (p=0.01). Also there was high correlation between TREC and RTE number (R=0.89; p<0.05). Estimation of TRECs is cheap, simple and fast method for identification of thymic output. It can be used in the cases when immunophenotyping is impossible and for neonatal screening of innate immune system defects.