Получение и исследование рекомбинантного виментина и его цитруллинированного аналога как антигена раннего ревматоидного артрита

Abstract

The major goal of this study was to obtain citrullinated vimentin that would be immunochemically identical to the protein recently isolated form a synovial fluid of patients with rheumatoid arthritis (RA). The procedure of obtaining recombinant vimentin and its citrullinated counterpart is described. When employed as a solid-phase capture antigen in our design of enzyme immunoassay, mutated citrullinated vimentin was fully competent in reaction with autoantibodies from serum of patients with verified early or advanced RA. The results demonstrate that citrullinated recombinant vimentin might provide a key reagent to design immunoassays with the potential for reliable diagnostics of early RA.