Влияние антимикробных пептидов медицинской пиявки Hirudo medicinalis на функциональную активность нейтрофилов

Abstract

Рассмотрены особенности действия новых антимикробных пептидов (АМП) медицинской пиявки на основные функциональные ответы нейтрофилов. Среди исследуемых АМП RWRVCFLCRRKKV (536_1) и FRIMRILRVLK (3967_1) обладали наибольшей способностью активировать респираторный взрыв нейтрофилов и усиливать дегрануляцию нейтрофилов, регистрируемую по экзоцитозу миелопероксидазы и лактоферрина.

The features of the action of new antimicrobial peptides (AMPs) of the medicinal leech on the main functional responses of neutrophils are considered. Among the studied AMPs RWRVCFLCRRKKV (536_1) and FRIMRILRVLK (3967_1) had the greatest ability to activate the respiratory burst of neutrophils and enhance neutrophil degranulation, recorded by exocytosis of myeloperoxidase and lactoferrin.

In the article, there are discussed the modern possibilities of laboratory diagnostics of alcohol abuse. There is given the characteristics of traditional indirect biomarkers with a narrow time window of detection, the level of which can increase in various pathological processes; direct biomarkers that react only to the intake of ethanol; a number of relatively new "candidates" for biomarkers or components of biomarker panels. Among all the used biomarkers, the most promising is the determination of phosphatidylethanol due to its high diagnostic sensitivity and specificity, as well as the ability to be included in the components of cell membranes, changing their structural and functional properties.

In the article, there are discussed the modern possibilities of laboratory diagnostics of alcohol abuse. There is given the characteristics of traditional indirect biomarkers with a narrow time window of detection, the level of which can increase in various pathological processes; direct biomarkers that react only to the intake of ethanol; a number of relatively new "candidates" for biomarkers or components of biomarker panels. Among all the used biomarkers, the most promising is the determination of phosphatidylethanol due to its high diagnostic sensitivity and specificity, as well as the ability to be included in the components of cell membranes, changing their structural and functional properties.