Effect of UV irradiation on fluorescence and antioxidant properties of γ-globulins

Abstract

Ultraviolet blood irradiation is an alternative approach for the treatment of infectious diseases of various etiologies and is a new immunomodulatory method. To date, there are no data in the literature on the precise mechanisms of the effects of UV radiation on various blood components, including proteins. The aim of the present work was to analyze the fluorescent properties of γ-globulins after UVI exposure (254 nm) with simultaneous registration of their antioxidant profile by chemiluminescent method in a model with alkylperoxyl radicals. We obtained that exposure of γ-globulins to UV leads to a dose-dependent decrease in fluorescence and a more complex kinetic curve of chemiluminescence due to the formation of products with stronger antioxidant properties.